Directional random oligonucleotide primed (DROP) global amplification of cDNA: its application to subtractive cDNA cloning
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چکیده
منابع مشابه
Directional random oligonucleotide primed (DROP) global amplification of cDNA: its application to subtractive cDNA cloning.
We describe a method of global PCR amplification of cDNA such that the strand sense is maintained. The products of this process are random primed fragments ranging in size from 100 to 500 bp which facilitates uniform PCR amplification of total cDNA. Directional incorporation of a T7 RNA polymerase initiator/promoter sequence allows efficient synthesis of total sense RNA from this material and t...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 1362-4962
DOI: 10.1093/nar/24.23.4832